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KMID : 0380020030180050352
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 5 p.352 ~ p.355
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Study on the Process to Decrease the Molecular Weight of β-[1,6]-branched β-[1,3]-D-Glucans
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Shin Hyun-Jae
Lee Dong-Cheol
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Abstract
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-(1,6)-Branched -(1,3)-D-glucans are known to enhance the immune system in human body, and in most cases have higher molecular weights over 1 MDa. In order to enhance the efficacy of glucans by decreasing their molecular weights, sonication, acid treatment, and enzymatic hydrolysis were tested and compared in this work. Treatment of sonication was effective to decrease the molecular weight to the extent of several dozens of kilo-daltons, but have a risk to disorder the triple helical structure of the glucans. Acid treatment was also an effective method to degrade polysaccharides, but -(1,6)-branched of the glucan molecules was found to be also hydrolyzed. Treatment of -(1,3)-glucanase was an effective method to decrease the molecular weight in mild conditions, but could not hydrolyse the highly -(1,6)-branched -(1,3)-glucans efficiently.
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KEYWORD
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Beta-glucan, sonication, acid treatment, enzymatic hydrolysis
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